Detecting TRK Fusions

TRK fusions can be detected with various testing methods 1,9

Next-generation sequencing (NGS)

  • Multigene parallel sequencing assay 9
  • Provides information about multiple biomarkers, including rare and common mutations, and can use less tissue 9,15
  • Can detect fusions in all 3 NTRK genes, as well as other genetic alterations 1
  • Can detect TRK fusion partner and position 1
  • Turnaround time for results may be longer than with IHC or FISH 16

Immunohistochemistry (IHC)

  • Uses specific antibodies to detect overexpression of the TRK fusion protein 9
  • Can be used to screen patients for TRK fusions, if included in panels 9
  • Requires dedicated tissue and limits multiplexing 15,17
  • Only detects the TRK component of the fusion protein; molecular confirmation is necessary 9

DNA fluorescence in situ hybridization (FISH)

  • Uses “break-apart” probes to show TRK gene rearrangement 9
  • Requires dedicated tissue 18
  • Delivers rapid results 16
  • Cannot distinguish fusion variants 16
  • May be labor intensive, possibly more expensive for multiple assays 9

Comprehensive molecular testing helps to uncover TRK fusion cancer. 1,9,10
Your pathologist can help you decide on an assay, such as NGS, that is right for your patient.

Double helix with people

TRK=tropomyosin receptor kinase.